Identification of soil-transmitted helminth eggs in students’ fingernails using the flotation method
Keywords:
soil-transmitted helminth infection, fingernails, elementary school students, personal hygiene, flotation methodAbstract
Soil-transmitted helminth infection remains a persistent public health concern, particularly among school-aged children who are frequently exposed to contaminated environments and may have suboptimal personal hygiene practices. This study aimed to identify the presence of soil-transmitted helminth eggs in students’ fingernails at an elementary school in Bali, Indonesia. A descriptive cross-sectional design was employed, involving 30 students selected through purposive sampling. Fingernail samples were collected and examined using the flotation method with saturated sodium chloride solution, followed by microscopic observation. The data were analyzed using descriptive statistics to determine the distribution of positive and negative findings. The results showed that a small proportion of samples were positive for helminth eggs, with only Ascaris lumbricoides identified, while the majority of samples were negative. These findings suggest that although fingernails can serve as a potential medium for helminth transmission, the level of contamination in this population appears relatively low. Nevertheless, the presence of positive cases indicates the continued importance of maintaining proper personal hygiene, particularly handwashing and regular nail care. This study highlights the relevance of simple preventive measures and supports the role of school-based hygiene education in reducing the risk of helminth infection among children.
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Aemiro, A., Menkir, S., Tegen, D., & Tola, G. (2022). Prevalence of Soil-Transmitted Helminthes and Associated Risk Factors Among People of Ethiopia: A Systematic Review and Meta-Analysis. Infectious Diseases, 15. https://doi.org/10.1177/11786337211055437
Agrawal, R., Pattnaik, S., Kshatri, J., Kanungo, S., Mandal, N., Palo, S., & Pati, S. (2024). Prevalence and correlates of soil-transmitted helminths in schoolchildren aged 5 to 18 years in low- and middle-income countries: a systematic review and meta-analysis. Frontiers in Public Health, 12. https://doi.org/10.3389/fpubh.2024.1283054
Amare, H., & Lindtjørn, B. (2021). Risk factors for scabies, tungiasis, and tinea infections among schoolchildren in southern Ethiopia: A cross-sectional Bayesian multilevel model. PLoS Neglected Tropical Diseases, 15. https://doi.org/10.1371/journal.pntd.0009816
Bista, C. B. (2023). Knowledge, Attitudes, and Practices of Hygiene in Hindu Society of Chunikhel, Lalitpur. Historical Journal. https://doi.org/10.3126/hj.v14i2.59045
Bokicho, B., Hailu, D., Eshetu, B., Matie, M., & Tadele, T. (2023). Soil-transmitted helminthiases among school-age children and their association with water, sanitation, and hygiene, Hawassa City, Southern Ethiopia. PLOS Neglected Tropical Diseases, 17. https://doi.org/10.1371/journal.pntd.0011484
Chen, J., Gong, Y., Chen, Q., Li, S., & Zhou, Y. (2024). Global burden of soil-transmitted helminth infections, 1990–2021. Infectious Diseases of Poverty, 13. https://doi.org/10.1186/s40249-024-01238-9
Danyati, N. P. N. T. (2018). Gambaran asuhan keperawatan penerapan TAK stimulasi persepsi defisit perawatan diri untuk mengatasi defisit perawatan diri pada pasien skizofrenia tahun 2018 [Poltekkes Kemenkes Denpasar]. https://repository.poltekkes-denpasar.ac.id/678/
Devi, N. P. A., Sudarmaja, I. M., & Swastika, K. (2018). Prevalensi infeksi soil transmitted helmith di Sekolah Dasar Negeri 1 Padangbulia Kecamatan Sukasada. Intisari Sains Medis, 9(3), 59–61. https://doi.org/10.1556/ism.v9i3.315
Dinas Kesehatan Provinsi Bali. (2020). Profil Kesehatan Provinsi Bali Tahun 2019. In Dinas Kesehatan Provinsi Bali. https://www.diskes.baliprov.go.id/download/profil-kesehatan-2019/
Eyayu, T., Yimer, G., Workineh, L., Tiruneh, T., Sema, M., Legese, B., Almaw, A., Solomon, Y., Malkamu, B., Chanie, E., Feleke, D., Jimma, M., Hassen, S., & Tesfaw, A. (2022). Prevalence, intensity of infection and associated risk factors of soil-transmitted helminth infections among school children at Tachgayint woreda, Northcentral Ethiopia. PLoS ONE, 17. https://doi.org/10.1371/journal.pone.0266333
Fitri, M. (2020). Analisis telur cacing soil transmitted helminthes pada kuku siswa sekolah dasar. Jurnal ’Aisyiyah Medika, 5(1), 131–141. https://doi.org/10.36729/jam.v5i1.319
Genet, A., Motbainor, A., Samuel, T., & Azage, M. (2021). Prevalence and associated factors of soil transmitted helminthiasis among school-age children in wetland and non-wetland areas of Blue Nile Basins, northwest Ethiopia: A community-based comparative study. SAGE Open Medicine, 9. https://doi.org/10.1177/20503121211063354
Gupta, S., Das, D., Chakraborty, A., & Banerjee, R. (2022). Factors predicting proper handwashing practice amidst the COVID-19 pandemic in India: a field-based cross-sectional study. Journal of Water and Health, 20 10, 1534–1542. https://doi.org/10.2166/wh.2022.103
Gurmassa, B. K., Gari, S., Solomon, E. T., Goodson, M., Walsh, C., Dessie, B., & Alemu, B. (2024). Prevalence and risk factors of soil transmitted helminths among vegetable farmers of Akaki river bank, Addis Ababa, Ethiopia. BMC Infectious Diseases, 24. https://doi.org/10.1186/s12879-024-09704-3
Irawati, O., & Sartini, I. F. (2021). Infeksi cacing nematoda usus pada anak kelas 1 dan 2 sekolah dasar. Jurnal Biologi Inovasi, 3(1), 1–7. https://doi.org/10.31289/jibioma.v3i1.538
Khan, K., Chakraborty, R., Brown, S., Sultana, R., Colón, A., Toor, D., Upreti, P., & Sen, B. (2021). Association between Handwashing Behavior and Infectious Diseases among Low-Income Community Children in Urban New Delhi, India: A Cross-Sectional Study. International Journal of Environmental Research and Public Health, 18. https://doi.org/10.3390/ijerph182312535
Lee, P., Kurscheid, J., Laksono, B., Park, M., Clements, A., Lowe, C., Stewart, D., & Gray, D. (2023). Model validation for a knowledge and practices survey towards prevention of soil-transmitted helminth infections in rural villages in Indonesia. Scientific Reports, 13. https://doi.org/10.1038/s41598-023-27781-3
Putri, P. H. J., & Subhaktiyasa, P. G. (2018). Hubungan Pengetahuan dengan Sikap Personal Hygiene Pemulung di Tempat Pembuangan Akhir Suwung Denpasar Selatan. Bali Medika Jurnal, 5(2), 292–297. https://doi.org/10.36376/bmj.v5i2.45
Rahmawati, Y., Harlita, T. D., & Yusran, D. I. (2023). Hubungan pengetahuan personal hygiene dengan correlation between knowledge of personal hygiene. Journal Health and Sciences, 8(1), 34–42. https://doi.org/10.35971/gojhes.v8i1.21725
Situmorang, P. R., Sihombing, R. A. K., & Hutabarat, Y. B. (2023). Identifikasi morfologi cacing STH (Soil Transmitted Helminth) pada kuku anak SD Yayasan Betania tahun 2023. Jurnal Kesehatan Tambusai, 4(3), 3152–3158. https://doi.org/10.31004/jkt.v4i3.17284
Speth, J. (2023). Guidelines in Practice: Hand Hygiene. AORN Journal, 118 2, 101–108. https://doi.org/10.1002/aorn.13964
Subhaktiyasa, P. G. (2024). Menentukan Populasi dan Sampel : Pendekatan Metodologi Penelitian Kuantitatif dan Kualitatif. Jurnal Ilmiah Profesi Pendidikan, 9(4), 2721–2731. https://doi.org/10.29303/jipp.v9i4.2657
Subhaktiyasa, P. G., Candrawati, S. A. K., Sumaryani, N. P., Sunita, N. W., & Syakur, A. (2025). Penerapan Statistik Deskriptif: Perspektif Kuantitatif dan Kualitatif. Emasains: Jurnal Edukasi Matematika Dan Sains, 14(1), 96–104. https://doi.org/10.59672/emasains.v14i1.4450
Suraini, S., & Oktavianti, V. (2019). Pemeriksaan Telur Cacing Soil Transmitted Helminths Pada Anak Usia 2-5 Tahun Di Nagari Batu Bajanjang Lembang Jaya Solok. Prosiding Seminar Kesehatan Perintis, 2(1), 117–122. https://jurnal.upertis.ac.id/index.php/PSKP/article/view/383
Tadege, B., Mekonnen, Z., Dana, D., Tiruneh, A., Sharew, B., Dereje, E., Loha, E., Ayana, M., & Levecke, B. (2022). Assessment of the nail contamination with soil-transmitted helminths in schoolchildren in Jimma Town, Ethiopia. PLoS ONE, 17. https://doi.org/10.1371/journal.pone.0268792
Tinungki, N. N., & Purnawinadi, G. (2023). Hubungan Kebersihan Diri dan Keluhan Gangguan Kulit Pada Petani. Nutrix Journal. https://doi.org/10.37771/nj.v7i2.1022
Widodo, A., & Ikawati, K. (2019). Pemeriksaan telur soil transmitted helminths pada kotoran kuku pemulung di tempat pembuangan akhir (TPA) sampah. Majalah Kesehatan Masyarakat Aceh (MaKMA), 2(2), 133–141. https://doi.org/10.32672/makma.v2i2.1303
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